human carcinoma epithelial lung a549 Search Results


human cancer cell lines a549  (ATCC)
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ATCC human cancer cell lines a549
Human Cancer Cell Lines A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonhepatic human lung carcinoma a549 cells  (CLS Cell Lines Service GmbH)
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CLS Cell Lines Service GmbH nonhepatic human lung carcinoma a549 cells
Nonhepatic Human Lung Carcinoma A549 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nsclc a549  (ATCC)
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ATCC human nsclc a549
Human Nsclc A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenocarcinomic human alveolar basal epithelial cell line  (ATCC)
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ATCC adenocarcinomic human alveolar basal epithelial cell line
Adenocarcinomic Human Alveolar Basal Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a549 cell culture tubes  (Diagnostic Hybrids Inc)
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Diagnostic Hybrids Inc a549 cell culture tubes
A549 Cell Culture Tubes, supplied by Diagnostic Hybrids Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adherence assays 2 12 1 a549 culture a549 human lung epithelial cell line  (ATCC)
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ATCC adherence assays 2 12 1 a549 culture a549 human lung epithelial cell line
Adherence Assays 2 12 1 A549 Culture A549 Human Lung Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a 549 human lung epithelial cells a 549  (DSMZ)
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DSMZ a 549 human lung epithelial cells a 549
A 549 Human Lung Epithelial Cells A 549, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wt p53  (ATCC)
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ATCC wt p53
Wt P53, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines  (ATCC)
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ATCC cell lines
Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human alveolar basal epithelial cells  (ATCC)
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ATCC human alveolar basal epithelial cells
Human Alveolar Basal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenocarcinoma epithelial cell line a549  (ATCC)
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ATCC adenocarcinoma epithelial cell line a549
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a549  (ATCC)
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ATCC a549
Cells were stained with (A) anti-α 1A ADR or (B) β 2 -ADR antibodies. After incubation, washing and centrifugation, alexa 488-conjugated gaot anit-rabbit IgG antibody was added and determined by flow cytometry (red filled region). PBS stained cells (blue lines) and secondary antibody stained cells (green lines) were showed as negative controls in each experiment. <t>A549,</t> RD, SK-N-SH, HL-60, THP-1, Jurkat and hPBMCs exhibited (A) α 1A - and (B) β 2 -ADRs. (C) The expression of β 2 -ADR on PMA-induced THP-1 cells were observed by immunofluorescent microscopy. The green fluorescence represented β 2 -adrenergic receptor. DAPI (blue) indicated cell nucleus. hPBMC, human peripheral blood mononuclear cells. The data were represented from three independent experiments.
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Cells were stained with (A) anti-α 1A ADR or (B) β 2 -ADR antibodies. After incubation, washing and centrifugation, alexa 488-conjugated gaot anit-rabbit IgG antibody was added and determined by flow cytometry (red filled region). PBS stained cells (blue lines) and secondary antibody stained cells (green lines) were showed as negative controls in each experiment. A549, RD, SK-N-SH, HL-60, THP-1, Jurkat and hPBMCs exhibited (A) α 1A - and (B) β 2 -ADRs. (C) The expression of β 2 -ADR on PMA-induced THP-1 cells were observed by immunofluorescent microscopy. The green fluorescence represented β 2 -adrenergic receptor. DAPI (blue) indicated cell nucleus. hPBMC, human peripheral blood mononuclear cells. The data were represented from three independent experiments.

Journal: PLoS ONE

Article Title: Norepinephrine and Epinephrine Enhanced the Infectivity of Enterovirus 71

doi: 10.1371/journal.pone.0135154

Figure Lengend Snippet: Cells were stained with (A) anti-α 1A ADR or (B) β 2 -ADR antibodies. After incubation, washing and centrifugation, alexa 488-conjugated gaot anit-rabbit IgG antibody was added and determined by flow cytometry (red filled region). PBS stained cells (blue lines) and secondary antibody stained cells (green lines) were showed as negative controls in each experiment. A549, RD, SK-N-SH, HL-60, THP-1, Jurkat and hPBMCs exhibited (A) α 1A - and (B) β 2 -ADRs. (C) The expression of β 2 -ADR on PMA-induced THP-1 cells were observed by immunofluorescent microscopy. The green fluorescence represented β 2 -adrenergic receptor. DAPI (blue) indicated cell nucleus. hPBMC, human peripheral blood mononuclear cells. The data were represented from three independent experiments.

Article Snippet: Cells of A549 (human lung carcinoma) (BCRC No. 60074; October 2008), RD (human rhabdomyosarcoma) (BCRC No. 60113; October 2008), SK-N-SH (human neuroblastoma) (ATCC No. HTB-11; December 2008), HL-60 (human promyelocytic leukemia) (BCRC No. 60027; March 2009), THP-1 (human monocytic leukemia) (BCRC No. 60430; October 2008), and Jurkat (human T cell leukemia) (BCRC No. 60424; January 2009) were maintained in Dulbecco's Modified Eagle Medium (DMEM) or RPMI 1640 medium (Life Technologies Gibco, USA) with heat-inactivated 10% fetal bovine serum (FBS) (Biological Industries, Israel) according to the instructions of the manufacturer [ , , ].

Techniques: Staining, Incubation, Centrifugation, Flow Cytometry, Expressing, Microscopy, Fluorescence

(A) A549 cells were infected with EV71 (MOI = 5) for 24 hrs and then treated with NE for 12 hrs. (B) SK-N-SH cells were infected with EV71 (MOI = 1) for 18 hrs and then treated with NE for 6 hours. (C, D) hPBMCs were infected with EV71 (MOI = 10) for 24 hrs and then treated with NE and EP for 24 hrs. The virus titers were significantly higher in (A) EV71-infected A549 cells and (B) SK-N-SH with various concentrations of NE than in EV71-infected cells without NE treatment. Both (C) NE and (D) EP elevated virus titers in EV71-infected hPBMCs at concentrations of 10 2 and 10 6 pg/mL, respectively. The data are shown as mean ± SEM of three independent experiments. White bar: virus only; black bar: virus plus NE or EP. *, P < 0.05; and **, P < 0.01 compared to virus only group and analyzed by one-way ANOVA and Post-Hoc Tukey test.

Journal: PLoS ONE

Article Title: Norepinephrine and Epinephrine Enhanced the Infectivity of Enterovirus 71

doi: 10.1371/journal.pone.0135154

Figure Lengend Snippet: (A) A549 cells were infected with EV71 (MOI = 5) for 24 hrs and then treated with NE for 12 hrs. (B) SK-N-SH cells were infected with EV71 (MOI = 1) for 18 hrs and then treated with NE for 6 hours. (C, D) hPBMCs were infected with EV71 (MOI = 10) for 24 hrs and then treated with NE and EP for 24 hrs. The virus titers were significantly higher in (A) EV71-infected A549 cells and (B) SK-N-SH with various concentrations of NE than in EV71-infected cells without NE treatment. Both (C) NE and (D) EP elevated virus titers in EV71-infected hPBMCs at concentrations of 10 2 and 10 6 pg/mL, respectively. The data are shown as mean ± SEM of three independent experiments. White bar: virus only; black bar: virus plus NE or EP. *, P < 0.05; and **, P < 0.01 compared to virus only group and analyzed by one-way ANOVA and Post-Hoc Tukey test.

Article Snippet: Cells of A549 (human lung carcinoma) (BCRC No. 60074; October 2008), RD (human rhabdomyosarcoma) (BCRC No. 60113; October 2008), SK-N-SH (human neuroblastoma) (ATCC No. HTB-11; December 2008), HL-60 (human promyelocytic leukemia) (BCRC No. 60027; March 2009), THP-1 (human monocytic leukemia) (BCRC No. 60430; October 2008), and Jurkat (human T cell leukemia) (BCRC No. 60424; January 2009) were maintained in Dulbecco's Modified Eagle Medium (DMEM) or RPMI 1640 medium (Life Technologies Gibco, USA) with heat-inactivated 10% fetal bovine serum (FBS) (Biological Industries, Israel) according to the instructions of the manufacturer [ , , ].

Techniques: Infection, Virus